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Detection and Characterization of PCR-SSCP Markers of the Bovine Lactoferrin Gene for Clinical Mastitis |
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Zhou Lei, Yuan-Yuan Yang, Zhong-Hao Li, Li-Juan Kong, Guan-Dong Xing, He-Shuang Di, Gen-Lin Wang* |
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Correspondence:
Gen-Lin Wang,
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| Abstract |
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A total of 80 cows, including 40 top mastitis resistant and 40 top mastitis susceptible animals as Group I and Group II, were selected from a population of 520 cows based on clinical mastitis occurrence. PCR-SSCP analysis on four fragments within the 5??region and two fragments of Exons 4,15 of bovine lactoferrin (bLF) revealed that four fragments-P1,P4,E4,E15-had polymorphisms which totally included six base mutations, and only two of them had significant differences in allele frequencies between resistant and susceptible groups, P1 (53.7% vs. 70.0%, p<0.05) and P4 (55.0% vs. 68.8%, p<0.05). Further study on these two promising markers combined with the milk performance traits of cows demonstrated that their selection would result in higher fat percentage (p<0.05), lower Somatic Cell Score (SCS) (p<0.05) and Clinical Mastitis Residuals (CMR) (p<0.01) indicating higher mastitis resistance and lower milk yield (p<0.05). The putative transcription factor binding sites in the 5??region were also studied by using MatInspector 7.2.2 software, and two signal pathways regulating the expression of bLF including the NF-??B pathway and nuclear hormone receptor pathway were predicted. |
| Keywords:
Cow; Lactoferrin; Clinical Mastitis; Polymorphism; PCR-SSCP |
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