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Animal Products
Asian-Australasian Journal of Animal Sciences 2005;18(12): 1794-1799.
https://doi.org/10.5713/ajas.2005.1794    Published online December 2, 2005.
Cholesterol Removal from Homogenized Milk with Crosslinked β-cyclodextrin by Adipic Acid
Eun-Mi Han, Song-Hee Kim, Joungjwa Ahn, Hae-Soo Kwak
The present study was carried out to develop crosslinking of 棺-cyclodextrin (棺-CD) using adipic acid, and to determine the optimum conditions of different factors (棺-CD concentration, mixing temperature, mixing time and mixing speed) on cholesterol reduction from homogenized milk. Crosslinked 棺-CD was prepared with adipic acid. When the milk was treated with different conditions, the cholesterol removal rate was in the range of 92.1 to 93.1% with 1% 棺-CD addition, which were not significantly different among treatments. After cholesterol removal from milk, the used crosslinked 棺-CD was washed for cholesterol dissociation and reused. For recycling study, the cholesterol removal rate in the first trial was 92.5%, which was mostly same as that using new crosslinked 棺- CD. With repeated ten time trials using same sample, 81.4% of cholesterol was removed from milk. Therefore, the present study indicated that the optimum conditions for cholesterol removal using crosslinked 棺-CD were 1% 棺-CD addition and 10 min mixing with 400 rpm speed at 5째C with over 90% cholesterol removal. In addition, crosslinked 棺-CD made by adipic acid resulted in the effective recycling efficiency.
Keywords: Crosslinked 棺-CD; Cholesterol Removal; Homogenized Milk; Recycling

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