Effect of Cellular Zinc on the Regulation of C2-ceramide Induced Apoptosis in Mammary Epithelial and Macrophage Cell Lines

Han, S. E.; Lee, H. G.; Yun, C. H.; Hong, Z. S.; Kim, S. H.; Kang, S. K.; Kim, S. H.; Cho, J. S.; Ha, S. H.; Choi, Y. J.

doi:2005.18.12.1741

Article

Ruminant Nutrition and Forage Utilization

Asian-Australasian Journal of Animal Sciences 2005;18(12): 1741-1745.
https://doi.org/10.5713/ajas.2005.1741 Published online December 2, 2005.

Effect of Cellular Zinc on the Regulation of C2-ceramide Induced Apoptosis in Mammary Epithelial and Macrophage Cell Lines

S. E. Han, H. G. Lee, C. H. Yun, Z. S. Hong, S. H. Kim, S. K. Kang, S. H. Kim, J. S. Cho, S. H. Ha, Y. J. Choi

Abstract

Zinc is a trace element that is associated with a stimulation of immune function and regulation of ion balance for livestock production. In this study, the effect of zinc as inhibitor to apoptosis-induced cells was examined in vitro using mammary epithelial cell line, HC11 and macrophage cell line, NCTC3749. Cell viability, measured by MTT assay, indicated that 10 g/ml of zinc had a negative impact on cellular activity and 50 ng/ml was chosen for further testing. Apoptosis was induced in cells treated with C2- ceramide in serum-free media. DNA fragmentation and gene expression of acidic sphingomyelinase (a gene responsible for the progress of apoptosis) were distinctively low in zinc treated cells compared with those in non-treated controls. In conclusion, zinc is involved in the regulation of cell proliferation and apoptosis in mammary epithelial cells and macrophages.

Keywords: Zinc; Cell Viability; Apoptosis; Immunity System