Newly Developed Microsatellite Markers of Mystus nemurus Tested for Cross-Species Amplification in Two Distantly Related Aquacultured Catfish Species

Chan, S. C.; Tan, S. G.; Siraj, S. S.; Yusoff, K.

doi:2005.18.11.1513

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2005;18(11): 1513-1518.
https://doi.org/10.5713/ajas.2005.1513 Published online December 2, 2005.

Newly Developed Microsatellite Markers of Mystus nemurus Tested for Cross-Species Amplification in Two Distantly Related Aquacultured Catfish Species

S. C. Chan, S. G. Tan, S. S. Siraj, K. Yusoff

Abstract

The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.

Keywords: Microsatellites; Cross-species Amplification; Mystus nemurus; Pangasius micronemus; Clarias batrachus