Relationship between Genetic Variants of Mitochondrial DNA  and Growth Traits in Hanwoo Cattle

Jeon, G. J.; Chung, H. Y.; Choi, J. G.; Lee, M. S.; Lee, C. W.; Park, J. J.; Ha, J. M.; Lee, H. K.; Sung, H. H.

doi:2005.18.3.301

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2005;18(3): 301-307.
https://doi.org/10.5713/ajas.2005.301 Published online April 19, 2005.

Relationship between Genetic Variants of Mitochondrial DNA and Growth Traits in Hanwoo Cattle

G. J. Jeon, H. Y. Chung, J. G. Choi, M. S. Lee, C. W. Lee, J. J. Park, J. M. Ha, H. K. Lee, H. H. Sung

Abstract

Genetic variants of Hanwoo mtDNA in the region of cytochrome oxidase subunit I, II and III complex were detected using restriction enzymes. PCR primers were designed based on the bovine mtDNA sequence, and 6 primer sets (Mt4, Mt5, Mt6, Mt7, Mt8 and Mt9) were used. A total of 20 restriction enzymes were used, and 6 restriction enzymes, which were Hinf I, Pvu II, Rsa I, Eco RI, Bgl II, and Msp I, showed genetic polymorphisms. Significant associations between genetic variants and weight traits were observed at WT15 (p<0.05) and WT18 (p<0.01) with Pvu II for Mt9, Bgl II for Mt6 and Rsa I for Mt8 segments in the region of cytochrome oxidase subunit complex. Significant associations were also observed at Mt9-Pvu II and Mt6-Bgl II segments for WT9 (p=0.01), WT12 (p=0.02), respectively. These results suggest that genetic variants of mtDNA in the region of cytochrome oxidase subunit complex may be candidate segments for improvement of animal growth as weight traits.

Keywords: mtDNA; Cattle; Polymorphism; Growth Traits