Calcium-binding Peptides Derived from Tryptic Hydrolysates of Cheese Whey Protein

Kim, S. B.; Lim, J. W.

doi:2004.17.10.1459

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2004;17(10): 1459-1464.
https://doi.org/10.5713/ajas.2004.1459 Published online January 1, 2004.

Calcium-binding Peptides Derived from Tryptic Hydrolysates of Cheese Whey Protein

S. B. Kim, J. W. Lim

Abstract

The purpose of this research was to investigate the potential use of cheese whey protein (CWP), a cheese by-product. The physiological activity of calcium-binding peptides in CWP may be used as a food additive that prevents bone disorders. This research also examined the characteristics of calcium-binding peptides. After the CWP was heat treated, it was hydrolyzed by trypsin. Then calcium-binding peptides were separated and purified by ion-exchange chromatography and reverse phase HPLC, respectively. To examine the characteristics of the purified calcium-binding peptides, amino acid composition and amino acid sequence were analyzed. Calcium-binding peptides with a small molecular weight of about 1.4 to 3.4 kDa were identified in the fraction that was flowed out from 0.25 M NaCl step gradient by ion-exchange chromatography of tryptic hydrolysates. The results of the amino acid analysis revealed that glutamic acid in a calcium-binding site took up most part of the amino acids including a quantity of proline, leucine and lysine. The amino acid sequence of calcium-binding peptides showed Phe-Leu-Asp-Asp-Asp-Leu-Thr-Asp and Ile-Leu-Asp-Lys from -LA and Ile-Pro-Ala-Val-Phe-Lys and Val-Tyr-Val-Glu-Glu-Leu-Lys from -LG.

Keywords: Cheese Whey Protein; Calcium-binding Peptide; Tryptic Hydrolysate