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Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes |
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C. S. Park, M. Y. Kim, Y. J. Yi, Y. J. Chang, S. H. Lee, J. J. Lee, M. C. Kim, D. I. Jin |
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| Abstract |
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The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4 C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant differences according to preservation day and incubation time, respectively. The sperm motility steadily declined from 96.9% at 0.5 h incubation to 78.8% at 6 h incubation at 1 day of preservation. However, the sperm motility rapidly declined after 4 day of preservation during incubation. The normal acrosome steadily declined from 93.3% at 0.5 h incubation to 73.8% at 6 h incubation at 1 day of preservation. However, the normal acrosome rapidly declined after 3 day of preservation during incubation. The rates of sperm penetration and polyspermy were higher in 5 and 10 106 sperm/ml than in 0.2 and 1 106 sperm/ml. Mean numbers of sperm in penetrated oocyte were highest in 10 106 sperm/ml compared with other sperm concentrations. The rates of blastocysts from the cleaved oocytes (2-4 cell stage) were highest in 1 106 sperm/ml compared with other sperm concentrations. In conclusion, we found out that liquid boar sperm stored at 4 C could be used for in vitro fertilization of pig oocytes matured in vitro. Also, we recommend 1횞106 sperm/ml concentration for in vitro fertilization of pig oocytes. |
| Keywords:
In vitro Fertilization; Pig Oocyte; Liquid Boar Sperm |
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