Establishment and Identification of a Debao Pony Ear Marginal Tissue Fibroblast Cell Line

Zhou, X. M.; Ma, Y. H.; Guan, W. J.; Zhao, D. M.

doi:2004.17.10.1338

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2004;17(10): 1338-1343.
https://doi.org/10.5713/ajas.2004.1338 Published online January 1, 2004.

Establishment and Identification of a Debao Pony Ear Marginal Tissue Fibroblast Cell Line

X. M. Zhou, Y. H. Ma, W. J. Guan, D. M. Zhao

Abstract

The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was successfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9 h and 48 h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.

Keywords: Debao Pony; Ear Marginal Tissue; Fibroblast Cell Line; Primary Explant Technique; Collagenase Technique