Isolation and Identification of Acid- and Bile-Tolerant Lactobacillus salivarius subsp. salivarius from Human Faeces

Bae, Hyoung Churl; Choi, Seong Hyun; Nam, Myoung Soo

doi:2001.14.8.1170

Article

Animal Products

Asian-Australasian Journal of Animal Sciences 2001;14(8): 1170-1178.
https://doi.org/10.5713/ajas.2001.1170 Published online August 1, 2001.

Isolation and Identification of Acid- and Bile-Tolerant Lactobacillus salivarius subsp. salivarius from Human Faeces

Hyoung Churl Bae, Seong Hyun Choi, Myoung Soo Nam

Abstract

This study was conducted to isolate lactobacilli having characteristics to be used as health adjuncts with fermented milk products. Acid tolerant strains were selected in Lactobacilli MRS broth adjusted to pH 4.0 from human faeces. Bile tolerant strains were examined in Lactobacilli MRS broth in which 1.0% bile salt was added. Microhemagglutination tests using swine erythrocytes were performed to select lactobacilli having adherence properties to survive in the intestinal tract. By examination of these characteristics the strain Nam 27, which was isolated from adult faeces, was selected and identified as Lactobacillus salivarius subsp. salivarius based on carbohydrate fermentation and 16S rDNA sequencing.

Keywords: Acid-; Bile-tolerant; Microhemagglutination; Probiotic; Lactobacillus salivarius