Effects of PGF2α and GnRH during Different Ovarian Status at Onset of Puberty in Murrah Buffalo Heifers (Bubalus bubalis) |
C. Singh, M. L. Madan |
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Abstract |
The objective of the investigation was to study the effect of intramuscular PGF2慣 and GnRH on estrus behavior and ovarian response in Murrah buffalo heifers. Twelve Murrah buffalo heifers at 32 months of age that had not exhibited behavioral estrus symptom were included in the experiment. Out of 12,4 heifers were in follicular phase (plasma estradiol 57.05 12.52 pg/ml), another 4 heifers were in luteal phase (Plasma progesterone 2.24 0.25 ng/ml) while the ovaries of remaining four heifers were inactive (estradiol 23.70 1.66 pg/ml and progesterone 0.32 0.06 ng/ml). PGF2慣 (25 mg, Lutalyse, im) and GnRH (200 ug, Fertagyl, iv) was administered to each heifer at interval of 10 days. The plasma progesterone concentration decreased within 48 hrs after PGF2慣 injection and followed thereafter with follicular growth, estrus and ovulation. GnRH administration induced follicular growth, elevation of plasma estradiol concentration with subsequent exhibition of behavioral estrus in 2 out of 4 heifers having inactive ovary. The observation reveals that Murrah buffalo heifers at 32 months of age have developed receptors for PGF2慣 and GnRH on ovarian and pituitary tissue respectively and response the single injection of PGF2慣 and GnRH similar to the mature cycling animals. |
Keywords:
PGF2慣; GnRH; Esturs; Buffalo Heifers |
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