Influence of Monensin and Virginiamycin on In Vitro Ruminal Fermentation of Ammoniated Rice Straw

Kook, K.; Sun, S. S.; Yang, C. J.; Myung, K. H.

doi:1999.12.4.544

Article

Ruminant Nutrition and Forage Utilization

Asian-Australasian Journal of Animal Sciences 1999;12(4): 544-547.
https://doi.org/10.5713/ajas.1999.544 Published online June 1, 1999.

Influence of Monensin and Virginiamycin on In Vitro Ruminal Fermentation of Ammoniated Rice Straw

K. Kook, S. S. Sun, C. J. Yang, K. H. Myung

Abstract

The object of this study was to determine the influence of monensin and virginiamycin (VM) on in vitro ruminal fermentation of rice straw or ammoniated rice straw. Rumen fluid was collected from 4 wethers fed 200g of concentrate supplement with 400g of untreated (U) or ammoniated (A) rice straw once daily for 28 days. Mixd ruminal microorganisms were incubated in anaerobic media that contained 20% (vol/vol) ruminal fluid and 0.3g of either U or A rice straw. Monensin and/or VM, dissolved in ethanol, were added in centrifuge tubes at final concentrations of 0, 15, 30, 15+15 and 30+30 ppm of culture fluid. The addition of monensin and VM combination to A rice straw fermentation decreased (p<0.05) the acetate to propionate ratio, total VFA and lactate production, but increased (p<0.05) pH. Total gas production tended to be decreased by the addition of monensin plus VM. Antimicrobial agents decreasd NH3 N concentration and dry matter digestibility.

Keywords: Monensin; Virginiamycin; In Vitro Fermentation; Ammoniated Rice Straw