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Comparative Study on the Effects of Combined Treatments of Lactic Acid Bacteria and Cellulases on the Cell Wall Compositions and the Digestibility of Rhodesgrass (Chloris gayana Kunth.) and Italian Ryegrass (Lolium multiflorum Lam.) Silages |
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M. Ridla, S. Uchida |
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| Abstract |
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This study was conducted to compare the effects of lactic acids bacteria (LAB) or LAB+cellulases on the cell wall composition and the in vitro dry matter digestibility (IVDMD) of Rhodesgrass (RG) and Italian ryegrass (IRG) silages. LAB (Lactobacillus cassei) at a concentration of 1.0횞105 cfu.g-1 fresh forage was added to all ensiling samples (except the untreated control) of RG and IRG. The cellulases used were Acremoniumcellulase (A), Meicelase (M) or a mixture of both (AM). Each cellulose was applied at levels of 0.005, 0.01 and 0.02% fresh sample. The samples were incubated at 20,30 and 40째C for about 2 months of storage. LAB inoculation did not affect cell wall components or IVDMD of both the RG and IRG silages, but LAB+cellulose treatments did. Increasing the amount of cellulose addition resulted in further decreases of cell wall concentrations. This reduction more markedly occurred with cellulases A and AM than it did with cellulose M. Cell wall components losses were higher in the IRG silages than in the RG silages. LAB+cellulose treatments decreased IVDMD of the RG silages, but had no effect on the IRG silages. The different effect of LAB+cellulose treatments on cell wall degradation and IVDMD of the RG and IRG silages suggested that RG contains more structural carbohydrates, which were difficult to degrade with cellulose, than did IRG. |
| Keywords:
Celllase; Cell Wall Composition; Italian Ryegrass; IVDMD; Lactic Acid Bacteria; Rhodesgrass |
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