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Hemagglutination and colony hybridization for the identification of enterotoxigenic Escherichia coli isolated from healthy pig |
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S. H. Choi, M. J. Oh, C. Sung |
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| Abstract |
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Erythrocytes from three different animal species were used to determine mannose-sensitive hemagglutination(MSHA) and mannose-resistant hemagglutination(MRHA) of 755 isolates obtained from rectal swabs of healthy pig. In addition, colony hybridization using digoxigenin-dUTP labeled polynucleotide probes was performed for the detection of heat-stable and heat-lavile enterotoxin genes carried by MRHA positive isolates. Of 755 strains, 9, 4 and 28 strains gave a positive MRHA with bovine, equine and pig erythrocytes, respectively. Of these isolates, 28(3.7%) were characterized for positive MRHA by at least one blood. Seven isolates gave a positive MRHA with two kinds of blood. Three gave a positive MRHA with three kinds of blood. Twenty-eight strains, while positive in MRHA, yielded negative signals in the colony hybridization assay for the detection of heat-stable(STaI and STAII) and geat-labile(LT) enterotoxin genes in E. coli. |
| Keywords:
MSHA; MRHA; Colony Hybridization; Enterotoxigenic E. coli |
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