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Effluent from Rusitec inoculated with rumen liquor or cow faeces as source s of micro-organisms for in vitro digestion of forage |
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S. Akhter, E. Owen, M. M. Hossain |
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| Abstract |
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The experiment investigated the possibility of using effluent from RUSITEC(rumen simulation technique) inoculated with rumen liquor or cow faeces as sources of micro-organisms for in vitro digestion of forages. Nine forages 횞 3 sources of inoculum were used in a factorial arrangement of treatments. Rumen liquor was collected from fistulated sheep and faeces was collected from cows. The RUSITEC apparatus consisted of 4 vessels, 2 vessels were charged with faecal liquor and 2 with rumen liquor. On the 8th day of the experiment RUSITEC effluent were collected to use in in vitro studies. In vitro OMD(g/kg) values using three sources of inoculum(fresh rumen liquor, RUSITEC effluent from rumen liquor or cow faeces) were statistically significant(p<0.001). The regression relationships between OMD using fresh rumen liquor and RISITEC effluent were highly significant(R2>0.90). The results suggest that RUSITEC effluent either from rumen liquor or cow faeces can be used as a source of micro-organisms for in vitro digestion of forages. |
| Keywords:
RUSITEC; Inoculum; In Vitro Digestion; Forages |
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