Gene transfer by manipulation of primordial germ cells in the chicken

Han, Jae Y.; Shohhner, R. N.; Guise, K. S.

doi:1994.7.3.427

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 1994;7(3): 427-434.
https://doi.org/10.5713/ajas.1994.427 Published online September 1, 1994.

Gene transfer by manipulation of primordial germ cells in the chicken

Jae Y. Han, R. N. Shohhner, K. S. Guise

Abstract

The primordial germ cells (PGCs) were transfected in vitro and expressed the exogenous RSVLTR/棺G2 plasmid, suggesting that PGC is a possible vector for direct gene transfer into the germ line. Transfection efficiency of cell suspensions containing PGGs was 1.5% by liposome mediated DNA transfection. By microinjection of the transfected PGCs into the host germinal crescent, PGCs migrated via blood vessel to the future gonad and these transfected PGCs resulted in the RSVLTR/棺G2 expression in the gonad. The results from the seeding of PGCs on the chorioallantoic membrane were insufficient to test the hypothesis that PGCs can penetrate or invade the chorioallantoic membrane for transport via the circulatory system.

Keywords: Transgenic Chicken; Primordial Germ Cells; Transfection; Microinjection; Plasmid