Go to Top Go to Bottom
Ruminant Nutrition and Forage Utilization
Asian-Australasian Journal of Animal Sciences 1991;4(2): 161-164.
DOI: https://doi.org/10.5713/ajas.1991.161    Published online June 1, 1991.
Determination of purine and pyrimidine bases in rumen micro-organisms by reversed phase HPLC after hydrolytic digestion under pressure
Y. K. Han, J. Landis
A rapid and accurate method is described for the determination of nucleo-bases in rumen microorganisms. A procedure to satisfactorily hydrolyse the micro-organisms involving reaction with a mixture of readily volatile organic acids (acetic and formic acids) under high pressure, is proposed. And optimal conditions for an analytical procedure with reversed phase HPLC is described. The following nucleobases contents (mmol/kg DM) of rumen micro-organisms were found: Adenine (Ade), 82.62; Guanine (Gua), 61.34; Cytosine (Cyt), 84.61; Thymine (Thy), 35.74; Uracil (Ura), 68.62; Hypoxanthine (Hxn), 13.06; Xanthine (Xn), 8.35. Total purine-N content (g/kg N) of rumen micro-organisms were 99.60. The nucleic acid N content (g/kg N) of microbial isolates were: RNA-N, 109.9; DNA-N, 50.9.
Keywords: Nucleobases; Purine-N; High Pressure Hydrolysis; HPLC
METRICS Graph View
  • 0 Crossref
  •  0 Scopus
  • 2,184 View
  • 6 Download
Related articles

Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : animbiosci@gmail.com               

Copyright © 2021 by Animal Bioscience. All rights reserved.

Close layer
prev next