Augmentation of Thermotolerance in Primary Skin Fibroblasts from a Transgenic Pig Overexpressing the Porcine HSP70.2

Chen, Ming-Yu; Tu, Ching-Fu; Huang, San-Yuan; Lin, Jyh-Hung; Tzang, Bor-Show; Hseu, Tzong-Hsiung; Lee, Wen-Chuan

doi:2005.18.1.107

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2005;18(1): 107-112.
https://doi.org/10.5713/ajas.2005.107 Published online April 19, 2005.

Augmentation of Thermotolerance in Primary Skin Fibroblasts from a Transgenic Pig Overexpressing the Porcine HSP70.2

Ming-Yu Chen, Ching-Fu Tu, San-Yuan Huang, Jyh-Hung Lin, Bor-Show Tzang, Tzong-Hsiung Hseu, Wen-Chuan Lee

Abstract

A high environmental temperature affects the economic performance of pigs. Heat shock protein 70 (HSP70) has been reported to participate importantly in thermotolerance. This study aims to produce transgenic pigs overexpressing porcine HSP70.2, the highly inducible one of HSP70 members, and to prove the cellular thermotolerance in the primary fibroblasts from the transgenics. A recombinant plasmid in which the sequence that encodes the porcine HSP70.2 gene is fused to green fluorescence protein (GFP) was constructed under the control of cytomegalovirus (CMV) enhancer and promoter. Two transgenic pigs were produced by microinjecting pCMV-HSP70-GFP DNA into the pronucleus of fertilized eggs. Immunoblot assay revealed the varied overexpression level (6.4% and 1.4%) of HSP70-GFP in transgenic pigs. After heating at 45째C for 3 h, the survival rate (78.1%) of the primary fibroblast cells from the highly expressing transgenic pig exceeded that from the non-transgenic pig (62.9%). This result showed that primary fibroblasts overexpressing HSP70-GFP confer cell thermotolerance. We suggest that transgenic pigs overexpressing HSP70 might improve their thermotolerance in summer and therefore reduce the economic loss in animal production.

Keywords: Heat Shock Protein 70.2; Transgenic Pig; Thermotolerance