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The Effects of Dimethyl-Sulfoxide Added to the Fertilization Medium on the Motility and the Acrosome Reaction of Spermatozoa and the Subsequent Development of Oocytes in Bovine |
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Y. Tsuzuki, D. H. Duran, M. Sawamizu, K. Ashizawa, N. Fujihara |
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| Abstract |
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This experiment was conducted to evaluate the influence of dimethyl-sulfoxise (DMSO, 0, 5, 50, 100 and 500 關M) on the motility and acrosome reaction of the frozen-thawed spermatozoa from 3 different bulls (Bull A, B and C). Also we evaluated the developmental capacity of bovine embryos fertilized in a medium containing DMSO at various concentrations. DMSO had negligible effects on the sperm motility and acrosome reaction in all three bulls. However, the development rates from 2 to 16 cells stage on the 3rd day after insemination with 50, 100 and 500 關M DMSO in Bull-B, and up to the blastocyst stage fertilized with 5, 50, 100 and 500 關M in Bull-A were significantly higher (p<0.05) than those of control (0 關M DMSO) group from each bull. Furthermore, the rates of blastocysts per cleaved embryos of 5 to 500 關M DMSO group in Bull-A and of 5 to 100 關M DMSO in Bull-C were also significantly higher (p<0.05) than those for their 0 關M groups, respectively. These results indicate that DMSO at micromol level used for in vitro fertilization might stimulate the development of embryos for some bulls. |
| Keywords:
DMSO; In Vitro Fertilization; Bovine Oocytes |
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