Anim Biosci > Volume 37(2); 2024 > Article |
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CONFLICT OF INTEREST
We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.
FUNDING
This project was financially supported by the Natural Science Foundation of China (32260921), the Jiangxi province natural science foundation of China (2020BAB205003), the Jiangxi province focuses on research and development plan (20171 BBF60008), and the China Agriculture Research System of MOF and MARA (CARS-37).
Items | Content (%) |
---|---|
Ingredients | |
Corn | 50.00 |
Cottonseed meal | 23.00 |
Wheat bran | 20.00 |
NaCl | 1.00 |
NaHCO3 | 1.00 |
Mineral–vitamin premix1) | 5.00 |
Total | 100 |
Nutrient levels2) | |
Dry matter | 84.16 |
Crude protein | 18.48 |
Crude fat | 3.53 |
Acid detergent fibre | 20.42 |
Neutral detergent fibre | 38.33 |
Calcium | 0.54 |
Total phosphorus | 0.74 |
Items | Supplemental levels of NA (mg/kg)1) | SEM | p-value | ||
---|---|---|---|---|---|
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CL | NA1 | NA2 | |||
OTU number | 1,163.67a | 952.67b | 1,130a | 27.32 | 0.04 |
Sobs | 1,281.70a | 1,046.30b | 1,238.70a | 26.21 | 0.04 |
Shannon | 5.54 | 5.33 | 5.53 | 0.06 | 0.32 |
Simpson | 0.02 | 0.02 | 0.01 | 0.003 | 0.49 |
Chao | 1,586.30a | 1,297.10b | 1,531.10a | 28.61 | 0.03 |
ACE | 1,590.20a | 1,308.20b | 1,556.90a | 25.09 | 0.02 |
Good’s coverage | 98.00 | 98.00 | 98.00 | 0.0003 | 0.43 |
NA, nicotinic acid; SEM, standard error of the mean value; OTU, operational taxonomic unit; Sobs, the observed species; ACE, abundance-based coverage estimator.
Item | Values of measurements1) | SEM | p-value | ||
---|---|---|---|---|---|
|
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CL | NA1 | NA2 | |||
pH value | 6.40 | 5.85 | 5.79 | 4.34 | 0.11 |
NH3-N (mg/100 mL) | 23.09 | 22.95 | 15.36 | 3.13 | 0.58 |
Acetate (mmol/L) | 51.47a | 69.42b | 73.82b | 0.89 | 0.01 |
Propionate (mmol/L) | 11.96 | 12.65 | 10.43 | 0.37 | 0.12 |
Butyrate (mmol/L) | 6.51a | 7.38a | 9.14b | 0.21 | 0.01 |
TVFA (mmol/L) | 69.92a | 89.44b | 93.39b | 1.21 | 0.01 |
Acetate/TVFA (%) | 73.66a | 77.61b | 79.03b | 0.34 | 0.01 |
Propionate/TVFA (%) | 17.06a | 14.14b | 11.14b | 0.26 | 0.01 |
Butyrate/TVFA (%) | 9.28 | 8.25 | 9.83 | 0.28 | 0.15 |
A/P | 4.33a | 5.49b | 7.13c | 0.13 | 0.01 |
Items | Values of apparent nutrient digestibility1) | SEM | p-value | ||
---|---|---|---|---|---|
|
|||||
CL | NA1 | NA2 | |||
DM (%) | 49.04a | 50.61a | 41.70b | 1.10 | 0.01 |
OM (%) | 51.50a | 53.62a | 44.99b | 1.14 | 0.02 |
CP (%) | 57.72 | 59.07 | 58.17 | 0.79 | 0.78 |
CF (%) | 59.38 | 56.29 | 53.22 | 1.57 | 0.29 |
ADF (%) | 41.92a | 47.13b | 46.33b | 0.65 | 0.01 |
NDF (%) | 47.50 | 44.08 | 40.77 | 1.35 | 0.17 |
DMI (kg/d)[13] | 10.52 | 10.84 | 10.72 | 0.07 | 0.15 |
NA, nicotinic acid; SEM, standard error of the mean; DM, dry matter; OM, organic matter; CP, crude protein; CF, crude fat; ADF, acid detergent fibre; NDF, neutral detergent fibre; DMI, dry matter intake.