Concentrations of Progesterone and Estradiol in Peripheral Plasma during the Estrous Cycle and after Ovariectomy in  Huanghuai Goats of High or Poor Prolificacy

Pang, X. S.; Wang, Z. Y.; Zhu, T. G.; Yin, D. Z.; Zhang, Y. L.; Meng, L.; Wang, F.

doi:2010.23.2.188

Article

Animal Reproduction and Physiology

Asian-Australasian Journal of Animal Sciences 2010;23(2): 188-196.
https://doi.org/10.5713/ajas.2010.90211 Published online December 22, 2009.

Concentrations of Progesterone and Estradiol in Peripheral Plasma during the Estrous Cycle and after Ovariectomy in Huanghuai Goats of High or Poor Prolificacy

X. S. Pang, Z. Y. Wang, T. G. Zhu, D. Z. Yin, Y. L. Zhang, L. Meng, F. Wang

Abstract

The objective of this study was to characterize the litter sizes in Huanghuai goats with high prolificacy (HP, five or more kids born per litter on at least one occasion), and to compare their peripheral blood concentrations of progesterone and estradiol with those of goats with poor prolificacy (PP, up to three kids born per litter on any occasion). The circulating concentrations of progesterone and estradiol were measured by radioimmunoassay from daily blood samples taken during natural estrus cycles and at 1-5 days after ovariectomy. Estrus was synchronized using two doses of a prostaglandin analog. Litter size for the HP goats increased up to a parity of five and decreased thereafter. The percentage of goats with litter sizes of ?? from parities 3 to 6 ranged from 44.5% to 58.3%. Although small differences in litter size were obtained for goats of parities three, four and six relative to five, parity five does had the highest mean litter size. Progesterone concentrations began to rise earlier and were higher in the HP than in the PP goats on most days of the luteal phase, but not during the follicular phase of the cycle or after ovariectomy. There was a significant difference between the two groups (p<0.05) in the magnitude of the progesterone plateau. Mean estradiol concentrations in the HP group remained significantly higher than in the PP group (p<0.05) during the estrus cycle. There were two estradiol peaks in the HP goats during the early luteal phase, but only one in the PP group. Measurements of individual corpora lutea (CL) in vitro showed that there was a greater prevalence of small CL (<6 mm in diameter) in the HP group than in the PP group (p<0.05). After ovariectomy, the estradiol level on day 1 was significantly higher than at the nadir during the estrus cycle in both the HP (p<0.01) and PP (p<0.05) goats, while levels decreased by 12.3% and 26.2% respectively compared with the mid-luteal period in HP and PP goats (p<0.05). The overall mean estradiol concentrations in HP goats were lower than in the PP group, but no significant differences were found between groups at 1-5 days after ovariectomy.

Keywords: Huanghuai Goat; Prolificacy; Progesterone; Estradiol; Estrous Cycle