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The effects of dimethyl-sulfoxide on the in vitro maturation and fertilization of bovine oocytes and the subsequent development |
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Y. Tsuzuki, D. H. Duran, Y. Kuroki, F. Uehara, K. Ashizawa, N. Fujihara |
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| Abstract |
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The present studies were undertaken to evaluate the effects of a low concentration of dimethyl-sulfoxide (DMSO) on in vitro maturation and development of bovine oocytes fertilized in vitro. Significantly more oocytes reached the metaphase stage of the second meiotic division in TCM-199 supplemented with 50 關M DMSO than in the control medium (p < 0.05), and the highest rates of development up to the blastocyst stage were obtained when 50 關M DMSO was added to the maturation and culture media (p < 0.05). The average of cell numbers of the blastocysts, expanded and hatched blastocysts cultured with 50 關M DMSO were 81.7, 125.7 and 129.9 cells, respectively. The proportion of blastocysts with normal chromosome numbers was 90.5%. These results suggest that the addition of 50 關M DMSO is beneficial for the maturation of bovine oocytes and production of the blastocysts with high quality. |
| Keywords:
DMSO; IVM; IVC; Bovine Oocytes; Embryos |
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