Role of Diacyl Glycerol (DAG) in Caprine Sperm Acrosomal Exocytosis Induced by Progesterone |
P. R. Somanath, K. K. Gandhi |
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Abstract |
Capacitated goat spermatozoa generated diacyl glycerol (DAG) when suspended in Krebs Ringer bicarbonate medium and induced by progesterone or Ca2+ ionophore A23187. We have added Sn-1-oleoyl-2-acetyl glycerol externally, to study the effect of DAG in goat sperm acrosomal exocytosis. Addition of neomycin abolished the DAG generating capacity of progesterone in a dose dependent manner, suggesting the involvement of a phosphoinositidase C activated phospholipase C system in the process. The level of increase in phosphatidic acid was considerably low and was produced well after the DAG generation thereby suggesting the involvement of a DAG kinase which phosphorylates DAG to produce PA. The inhibition of progesterone mediated effect by inhibitors of GABAA/Cl channel and Ca2+ channels further supports the evidence that the events of binding of agonist to the receptor(s), opening of Ca2+ channels and the activation of phospholipase C are reconciled to perform the function of acrosome reaction in capacitated goat spermatozoa. |
Keywords:
Goat; Spermatozoa; Acrosome Reaction; Diacyl Glycerol; Phospholipase C |
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