Influence of Antisense IGFBP-2 Oligo Deoxynucleotide Administration on Tissue IGFBP-2 Gene Expression in Chicks

Nagao, K.; Osada, K.; Murai, A.; Okumura, J.; Kita, K.

doi:2001.14.12.1781

Article

Animal Products

Asian-Australasian Journal of Animal Sciences 2001;14(12): 1781-1784.
https://doi.org/10.5713/ajas.2001.1781 Published online December 1, 2001.

Influence of Antisense IGFBP-2 Oligo Deoxynucleotide Administration on Tissue IGFBP-2 Gene Expression in Chicks

K. Nagao, K. Osada, A. Murai, J. Okumura, K. Kita

Abstract

We have examined the influence of antisense oligo deoxynucleotide (ODN) of IGFBP-2 on tissue IGFBP-2 gene expression in chicks. Antisense IGFBP-2 ODN was directly injected into the liver or cerebroventricle. Control birds were injected with vehicle. The hepatic IGFBP-2 gene expression was decreased to approximately 30% of the control at 2 h after injection of antisense ODN. In the brain of chickens injected with antisense ODN, IGFBP-2 mRNA level did not change after 2 h of injection and decreased to approximately 60% of the control after 6 h of injection. These results showed that the expression of IGFBP-2 gene in the liver and brain was successfully suppressed by administrating antisense ODN and that hepatic IGFBP-2 gene expression was quickly suppressed by antisense ODN compared with the brain.

Keywords: Chickens; Antisense; IGFBP-2; Liver; Brain